Fungi can produceĭifferent types of enzymes extracellularly, which gives a great chance for producing in large amounts

Recently,įungi have been used for the production of more than 50% of the needed enzymes. Technology in products manufacturing ranging from tea and coffee to fruit juice and wine. Used in other fields such as paper production, detergent, the textile industry, and in drinks and food Production, and commodities manufacturing, such as linen and leather. Fungal enzymes have been usedįrom ancient times to today in many industries, including baking, brewing, cheese making, antibiotics Products suitable shelf life, affordable cost, and approved demands. Fungal enzymes have appropriate stability to give manufactured Proper products for many uses in medicinal requests, industrial processing, bioremediation purposes,Īnd agricultural applications. Fungal enzymes are compatible, efficient, and They are proteins that break down and convertĬomplicated compounds to produce simple products. marcescens HK2 could be a promising candidate for foods and biofuels industries.Įnzymes have played a crucial role in mankind’s challenges to use different types ofīiological systems for a diversity of applications. The strain has an ability to utilize low cost agricultural residue and the whole cell immobilization of strain improved the enzyme production which can further minimize the cost of down streaming processing. The strain HK2 was identified as the Serratia marcescens by 16S rRNA gene sequencing. SDS-PAGE of crude enzyme revealed that the molecular weight of xylanase and GI were about 55 kDa and 63 kDa respectively. The GI activity was 3.3 folds higher than control when 1.5% xylose and 1:3 (w/w) ratio of peptone and yeast extract were used in the culture medium. Similarly, the strain preferred 35 to 40 ☌ temperature and 8 to 9 pH for efficient GI production. The strain produced highest xylanase activity of 23.09 ± 0.009 U/ml when grown in beechwood xylan and yeast extract in a ratio of 3:2 (w/w) at 37 ☌ and pH 7 for 96 h. The qualitative enzymatic assay of strain HK2 in xyan agar and xylose agar plates showed a zone of clearance which indicated that the strain has potential to produce xylanase and xylose/glucose isomerase (GI) respectively. The optimization of fermentation parameters for higher yield of bacterial enzymes have significant contribution to the industries. The optimum pH for best enzyme activity was 6.0 while optimum temperature for enzyme production was 50 oC. Best enzyme activity was observed in the presence of Mg2+ (specific activity= 6.85 U/mg protein) while Hg2+ was inhibitory (specific activity= 1.02 U/mg protein). In general, organic nitrogen substrates supported higher enzyme productivity than inorganic nitrogen substrates. Lowest enzyme activity was observed with sodium trioxonitrate (specific activity = 2.44 U/mg protein). The effects of nitrogen substrates on glucose isomerase production showed that yeast extract supported maximum enzyme activity (specific activity= 5.24 U/mg protein). Media containing only xylose or glucose gave lower enzyme productivies (specific activities= 4.60 and 2.35 U/mg protein respectively). Enzyme was produced using a variety of carbon substrates but the highest enzyme activity was detected in a medium containing 0.5% xylose and 1 % glycerol (specific activity = 6.88 U/mg protein).

The bacterium was selected based on the release of 3.62 mg/mL fructose from the fermentation of glucose. Please click on a product title for detailed information and picture, or browse the Categories page.This work reports the effects of some culture conditions on the production of glucose isomerase by Bacillus licheniformis. You can scroll down to view the inventory or use Search box to search for keywords. This page represents our entire inventory sorted by manufacturer#8217 s name.

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